The systematic analysis of culture success rate of our optimized culture protocol, reveals that 37% of all gliomas cannot be cultured under serum-free conditions. These are predominantly grade II,III and secondary grade IV gliomas.
Alternative in vitro models are being developed for growth or sustainment of low-grade glioma, including various 3D model systems. In addition, alternative culture conditions are being evaluated to support growth of a specific subpopulation of high grade glioma for which current protocols are not suitable.